A new method for spectrophotometric determination of colchicoside
نویسنده
چکیده
A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are linear over 25.00-50.00 μgml, 22.00-38.00 μgml and apparent molar absorptivity is calculated to be 2.28 x 10 l mol cm and 2.77 x 10 l mol cm for CAS-AB and CAS-SO respectively. Method is successfully applied to assay of COLSID in pharmaceutical formulations.
منابع مشابه
Novel methods for the spectrophotometric determination of colchicoside
Two novel methods are presented for the spectrophotometric determination of colchicoside (COLSID). Method A involves the reaction of COLSID with Fe(III) to form complex and method B is based on the reaction of the drug with NH2OH.HCl, the absorbances of which are measured at 458 nm and 395 nm respectively. The calibration graphs are found to be linear over 20.00-120.00 and 20.00-100.00 μgmL for...
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